Discovery of a chemical compound that suppresses expression of BEX2, a dormant cancer stem cell-related protein

Cancer stem cells (CSCs) are believed to cause cancer metastasis and recurrence. BEX2 (brain expressed X-linked gene 2) is a CSC-related that in dormant CSCs cholangiocarcinoma induces resistance against chemotherapy. The aim of the present study was identify small compounds have activity inhibit expression result attenuation phenotypes. We screened 9600 chemical high-throughput screening using cell line HuCCT1 expressing protein fused with NanoLuc, identified compound, BMPP (1, 3-Benzenediol, [4-(4-methoxyphenyl)-1H-pyrazol-3-yl]). found exert decreasing effects on G0 phase population tumor cells, increasing ATP levels chemotherapeutic sensitivity cells. These findings indicate valuable compound for reducing Thus, identification as potential CSC suppressor provides scope development novel therapeutic modalities treatment cancers overexpressing CSCs.